psin4 e2f o2s (Addgene inc)

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Addgene inc psin4 e2f o2s
Psin4 E2f O2s, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/psin4+ef2+o2s/pSIN4-EF2-O2S+(Plasmid+%2321162)/bio_rxiv__2025__11__27__690966-149-9-10
Average 93 stars, based on 26 article reviews

psin4 e2f o2s - by Bioz Stars, 2026-10

93/100 stars

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CRISPR:

Article Title: A chromatin-focused CRISPR screen identifies USP22 as a barrier to somatic cell reprogramming.
Article Snippet: .. CRISPR-infected cells were reseeded as 50.000 cells and infected with lentiviral reprogramming factors (pSIN4-CMV-K2M (Addgene plasmid #21164) and pSIN4-EF2-O2S (Addgene plasmid 21162)72; 100–250 μl supernatant/virus was used per well (determined empirically for each batch of virus) in a medium containing 8 μg/ml protamine sulfate. ..

Infection:

Article Title: BRD9-containing non-canonical BAF complex maintains somatic cell transcriptome and acts as a barrier to human reprogramming
Article Snippet: .. For human naïve-like reprogramming, dH1f cells were infected with pSIN4-EF2-O2S (Addgene #21162), pSIN4-CMV-K2M (Addgene #21164), pLOVE-miR302/367 (Addgene #31310) and pSIN2-EF2-Nanog-Pur (Addgene #16758). ..

Plasmid Preparation:

Article Title: Human Pluripotency Is Initiated and Preserved by a Unique Subset of Founder Cells.
Article Snippet: .. Lentiviral vectors used are: pSIN4-EF2-O2S, pSIN4-EF2K2M (gifts from Dr. James Thomson; Addgene plasmid #21162 and #21164), pEGIP (a gift from Linzhao Cheng; Addgene plasmid #26777), modified pLV-H1TetO-RFP-Puro (Biosettia #SORT-C02; described below), and TRC lentiviral shRNA vectors (Table S3). .. Fifty-thousands adult human dermal fibroblasts (ScienCell) at passage#5 or human newborn foreskin BJ fibroblasts (ATCC) were seeded with 0.8 mL Fibroblast Medium (DMEM supplemented with 10% FBS and 1 3 Non-essential amino acids) per well on a e5 Cell 177, 1–15.e1–e10, May 2, 2019 Please cite this article in press as: Nakanishi et al., Human Pluripotency Is Initiated and Preserved by a Unique Subset of Founder Cells, Cell (2019), https://doi.org/10.1016/j.cell.2019.03.013 12-well plate.

Virus:

Modification:

shRNA:

Cell Culture:

Article Title: Derivation of Induced Pluripotent Stem Cells by Lentiviral Transduction
Article Snippet: .. Bacterial Cell Culture Bacterial stabs: pSIN4-EF2-O2S (Addgene, #21162). .. OCT4 and SOX2 genes. pSIN4-EF2-N2L (Addgene, #21163).

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